Galectin-1 Antibody (GAL1/1831)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53212
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0002.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]")
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0006.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Lambda Clone # GAL1/1831
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa12-108) of human Galectin-1 protein (exact sequence is proprietary) (Uniprot: P09382)
Localization
Secreted (extracellular matrix)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Lambda
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54457)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Galectin-1 Antibody (GAL1/1831)
Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]
Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).![Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-1831-Immunocytochemistry-Immunofluorescence-NBP2-53212-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]")
Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]
Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Confocal immunofluorescence image of HeLa cells using Galectin-1 Antibody (GAL1/1831). Green (CF488) and Red Dot is used to label the nuclei Red.![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-1831-Immunohistochemistry-Paraffin-NBP2-53212-img0001.jpg "Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]")
Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]
Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0005.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]")
![Protein Array: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-1831-Protein-Array-NBP2-53212-img0004.jpg "Protein Array: Galectin-1 Antibody (GAL1/1831) [NBP2-53212]")
Applications for Galectin-1 Antibody (GAL1/1831)
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Galectin-1
Additional Galectin-1 Products
Product Documents for Galectin-1 Antibody (GAL1/1831)
Product Specific Notices for Galectin-1 Antibody (GAL1/1831)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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