Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79855
Recombinant Monoclonal Antibody
![Protein Array: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Protein-Array-NBP2-79855-img0001.jpg "Protein Array: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]")
![Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Western-Blot-NBP2-79855-img0004.jpg "Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # GAL1/2499R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa 12-108) of human Galectin-1 protein (exact sequence is proprietary) (Uniprot: P09382)
Localization
Secreted (extracellular matrix)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79721).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]
Western Blot: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Western Blot Analysis of HeLa cell lysate using Galectin-1 antibody (GAL1/2499R).![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79855-img0002.jpg "Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]")
Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]
Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Galectin-1 Antibody (GAL1/2499R).![Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Western-Blot-NBP2-79855-img0003.jpg "Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]")
Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]
Western Blot: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/2499R).Applications for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Galectin-1
Alternate Names
BHL, Galaptin, Galectin1, GBP, L-14, LGALS1
Gene Symbol
LGALS1
Additional Galectin-1 Products
Product Documents for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Product Specific Notices for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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