Galectin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16580

Novus Biologicals, part of Bio-Techne
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NBP2-16580
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Galectin-1 Antibodies »

Product Summary for Galectin-1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin-1. The exact sequence is proprietary.

Reactivity Notes

Bovine (85%), Dog (81%), Pig (84%), Sheep (84%).

Localization

Secreted; extracellular space; extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Galectin-1 Antibody

Western Blot: Galectin-1 Antibody [NBP2-16580]

Western Blot: Galectin-1 Antibody [NBP2-16580]

Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16580]

Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16580]

Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16580] - Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 1 stained by Galectin 1 antibody diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Frozen: Galectin-1 Antibody [NBP2-16580]

Immunohistochemistry-Frozen: Galectin-1 Antibody [NBP2-16580]

Immunohistochemistry-Frozen: Galectin-1 Antibody [NBP2-16580] - Frozen sectioned E13.5 Rat brain. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
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Applications for Galectin-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Galectin-1

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Gene Symbol

LGALS1

UniProt

P09382

Additional Galectin-1 Products

Product Documents for Galectin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Galectin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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