Galectin-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89795

Novus Biologicals, part of Bio-Techne
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NBP1-89795
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Galectin-3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Galectin-3 Antibody

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795]

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795]

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining in human colon and liver tissues using NBP1-89795 antibody. Corresponding LGALS3 RNA-seq data are presented for the same tissues.
Western Blot: Galectin-3 Antibody [NBP1-89795]

Western Blot: Galectin-3 Antibody [NBP1-89795]

Western Blot: Galectin-3 Antibody [NBP1-89795] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-LGALS3 antibody. Corresponding LGALS3 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795]

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795]

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
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Applications for Galectin-3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Galectin-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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