Galectin-4 Antibody (9Z2B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16249

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16249-100ul
NBP3-16249-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Z2B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Galectin-4 Antibodies »

Product Summary for Galectin-4 Antibody (9Z2B5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin-4 (P56470). MAYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDGWDKVVFNTLQGGKWGSEERKRSMPFKKGAA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Galectin-4 Antibody (9Z2B5)

Western Blot: Galectin-4 Antibody (9Z2B5) [NBP3-16249]

Western Blot: Galectin-4 Antibody (9Z2B5) [NBP3-16249]

Western Blot: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Western blot analysis of extracts of various cell lines, using Galectin-4 Rabbit mAb (NBP3-16249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249]

Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249]

Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded human appendix using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249]

Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249]

Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded mouse colon using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Galectin-4 Antibody (9Z2B5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galectin-4

Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.

Alternate Names

GAL4, Galectin4, LGALS4

Gene Symbol

LGALS4

Additional Galectin-4 Products

Product Documents for Galectin-4 Antibody (9Z2B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Galectin-4 Antibody (9Z2B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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