GALK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81853

Novus Biologicals, part of Bio-Techne
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NBP1-81853
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GALK1 Antibodies »

Product Summary for GALK1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GEHTDYNQGLVLPMALELMTVLVGSPRKDGLVSLLTTSEGADEPQRLQFPLPTAQRSLEPGTPRWANYVKGVIQYYPAAPLPGFSAVVVSSVPLGGGLSSSASLEVATYTFLQQLCPDSGTIAARAQVCQQAEHSFAGMPCGIMDQFI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GALK1 Antibody

Western Blot: GALK1 Antibody [NBP1-81853]

Western Blot: GALK1 Antibody [NBP1-81853]

Western Blot: GALK1 Antibody [NBP1-81853] - Analysis using Anti-GALK1 antibody NBP1-81853 (A) shows similar pattern to independent antibody NBP1-81854 (B).
Immunocytochemistry/ Immunofluorescence: GALK1 Antibody [NBP1-81853]

Immunocytochemistry/ Immunofluorescence: GALK1 Antibody [NBP1-81853]

Immunocytochemistry/Immunofluorescence: GALK1 Antibody [NBP1-81853] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: GALK1 Antibody [NBP1-81853]

Immunohistochemistry-Paraffin: GALK1 Antibody [NBP1-81853]

Immunohistochemistry-Paraffin: GALK1 Antibody [NBP1-81853] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
View 1 more images

Applications for GALK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALK1

The GALK1 gene encodes for a major enzyme involved in the metabolism of galactose. The protein coded is 392 amino acids long at a mass of around 42 kDA. A lack of the protein coded by the GALK1 gene results in infantile congenital cataracts and adult presenile cataracts. The GALK1 gene has also been linked to pneumonia, jaundice, hepatoblastoma, citrin deficiency, galactosemia, down syndrome, liver disease, ovarian cancer, and mevalonic aciduria. It interacts with DMWD, OTUD4, ATG2A, ATG10, AGO4 to function in galactose metabolism, the Leloir pathway, and colanic acid building blocks biosynthesis.

Long Name

Galactokinase 1

Alternate Names

GALK, HEL-S-19

Gene Symbol

GALK1

Additional GALK1 Products

Product Documents for GALK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GALK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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