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GALT Antibody (JE64-66)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32362

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32362

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE64-66

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within human GALT aa 41-90/379. (Uniprot: P07902)

Localization

Cytosol, Golgi apparatus, cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GALT Antibody (JE64-66)

GALT Antibody (JE64-66)

Western Blot: GALT Antibody (JE64-66) [NBP3-32362]

Western Blot: GALT Antibody (JE64-66) [NBP3-32362] - Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (NBP3-32362) at 1/500 dilution.

Lane 1: Hela cell lysate (10 ug/Lane)
Lane 2: HepG2 cell lysate (10 ug/Lane)
Lane 3: Rat brain tissue lysate (20 ug/Lane)
Lane 4: Mouse kidney tissue lysate (20 ug/Lane)

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32362) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
GALT Antibody (JE64-66)

Immunohistochemistry: GALT Antibody (JE64-66) [NBP3-32362]

Immunohistochemistry: GALT Antibody (JE64-66) [NBP3-32362] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-GALT antibody (NBP3-32362) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32362) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for GALT Antibody (JE64-66)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:400

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALT

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Alternate Names

EC 2.7.7.12, Gal-1-P uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene Symbol

GALT

Additional GALT Products

Product Documents for GALT Antibody (JE64-66)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GALT Antibody (JE64-66)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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