GALT Antibody (PSH02-88)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32364
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH02-88
Concentration
1 mg/ml
Product Summary for GALT Antibody (PSH02-88)
Immunogen
Recombinant protein within human GALT aa 1-379 / 379. (Uniprot: P07902)
Localization
Cytosol, Golgi apparatus, cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GALT Antibody (PSH02-88)
Western Blot: GALT Antibody (PSH02-88) [NBP3-32364]
Western Blot: GALT Antibody (PSH02-88) [NBP3-32364] - Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (NBP3-32364) at 1/5,000 dilution.Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: K-562 cell lysate (15 µg/Lane)
Lane 5: THP-1 cell lysate (15 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time:
Lane 1-5 (left): 1 minute 34 seconds;
Lane 1-5 (right): 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for GALT Antibody (PSH02-88)
Application
Recommended Usage
Western Blot
1:2000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GALT
Alternate Names
EC 2.7.7.12, Gal-1-P uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase
Gene Symbol
GALT
Additional GALT Products
Product Documents for GALT Antibody (PSH02-88)
Product Specific Notices for GALT Antibody (PSH02-88)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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