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GAMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54399

Novus Biologicals, part of Bio-Techne
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NBP1-54399

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Summary for GAMT Antibody

Immunogen

Synthetic peptides corresponding to GAMT(guanidinoacetate N-methyltransferase) The peptide sequence was selected from the middle region of GAMT. Peptide sequence PGEGPFLTPWVGWTVLVHLEIKVLCLAQWLPGAVAQVYNPSTVEGRGGQI. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for GAMT Antibody

Western Blot: GAMT Antibody [NBP1-54399]

Western Blot: GAMT Antibody [NBP1-54399]

Western Blot: GAMT Antibody [NBP1-54399] - Antibody Titration: 1 ug/ml Human liver.
Immunohistochemistry: GAMT Antibody [NBP1-54399]

Immunohistochemistry: GAMT Antibody [NBP1-54399]

Immunohistochemistry: GAMT Antibody [NBP1-54399] - Human Adult liver Observed Staining: Cytoplasmic Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 secProtocol located in Reviews and Data.
Western Blot: GAMT Antibody [NBP1-54399]

Western Blot: GAMT Antibody [NBP1-54399]

Western Blot: GAMT Antibody [NBP1-54399] - Human Thymus lysate, concentration 0.2-1 ug/ml.

Applications for GAMT Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAMT

GAMT is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in its gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumula

Alternate Names

EC 2.1.1.2, guanidinoacetate N-methyltransferase, PIG2, TP53I2

Gene Symbol

GAMT

UniProt

Additional GAMT Products

Product Documents for GAMT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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