Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GAMT Antibodies »

Product Summary for GAMT Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Guanidinoacetate N-methyltransferase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAMT Antibody

Western Blot: GAMT Antibody [NBP2-34854]

Western Blot: GAMT Antibody [NBP2-34854]

Western Blot: GAMT Antibody [NBP2-34854] - Detection of Human GAMT by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells.
Immunoprecipitation: GAMT Antibody [NBP2-34854]

Immunoprecipitation: GAMT Antibody [NBP2-34854]

Immunoprecipitation: GAMT Antibody [NBP2-34854] - Detection of Human GAMT by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells.

Applications for GAMT Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Validation by IP/Western Blot was performed using a Western Blot Gel 4-20%, 10 x 10 cm.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAMT

GAMT is encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene.

Alternate Names

EC 2.1.1.2, guanidinoacetate N-methyltransferase, PIG2, TP53I2

Entrez Gene IDs

2593 (Human)

Gene Symbol

GAMT

UniProt

NP_000147.1 (Human)

Additional GAMT Products

Product Documents for GAMT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GAMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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