Gankyrin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1834
![Western Blot: Gankyrin Antibody [NB100-1834]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-Western-Blot-NB100-1834-img0003.jpg "Western Blot: Gankyrin Antibody [NB100-1834]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.97 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human Gankyrin protein sequence (between residues 100-200). [UniProt# O75832]
Reactivity Notes
Predicted to react with mouse, rat, porcine, bovine, and zebrafish based on 100% sequence homology.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Gankyrin Antibody
Western Blot: Gankyrin Antibody [NB100-1834]
Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.![Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NB100-1834]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1834-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NB100-1834]")
Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NB100-1834]
Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NB100-1834] - Gankryrin antibody (1:500) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.![Flow (Intracellular): Gankyrin Antibody [NB100-1834]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-Flow-Intracellular-NB100-1834-img0009.jpg "Flow (Intracellular): Gankyrin Antibody [NB100-1834]")
Flow (Intracellular): Gankyrin Antibody [NB100-1834]
Flow (Intracellular): Gankyrin Antibody [NB100-1834] - Intracellular staining of Jurkat cells (1 x 10^6 cells/ml) with Gankyrin antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).Applications for Gankyrin Antibody
Application
Recommended Usage
Flow (Intracellular)
1:500
Immunocytochemistry/ Immunofluorescence
1:500
Western Blot
2.0 ug/ml
Application Notes
This Gankyrin antibody is useful in Western blot, where a band is observed ~24 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.97 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Gankyrin
Alternate Names
26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, ankyrin repeat protein, dJ889N15.2, Gankyrin, hepatocellular carcinoma-associated protein p28-II, p28, p28(GANK), proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
Gene Symbol
PSMD10
Additional Gankyrin Products
Product Documents for Gankyrin Antibody
Product Specific Notices for Gankyrin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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