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GAP-43 Antibody (3H14)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05535

Novus Biologicals, part of Bio-Techne
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NBP3-05535-100ul
NBP3-05535-50ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 3H14

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length Human GAP-43

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAP-43 Antibody (3H14)

Western Blot: GAP-43 Antibody (3H14) [NBP3-05535]

Western Blot: GAP-43 Antibody (3H14) [NBP3-05535]

Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (3H14) [NBP3-05535]

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (3H14) [NBP3-05535]

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP3-05535] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with GAP-43 Antibody at a dilution of 1:1,000, in red, and costained with chicken pAb to MAP2 at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP-43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.

Applications for GAP-43 Antibody (3H14)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000

Immunohistochemistry

1:1000-5000

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody (3H14)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAP-43 Antibody (3H14)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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