GAP-43 Antibody (CL10847) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44282
![GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]](https://resources.bio-techne.com/images/products/nbp3-44282_mouse-gap-43-mab-cl10847-azide-and-bsa-free-282202513121842.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]")
![GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]](https://resources.bio-techne.com/images/products/nbp3-44282_mouse-gap-43-mab-cl10847-azide-and-bsa-free-28220251344229.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]")
![GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]](https://resources.bio-techne.com/images/products/nbp3-44282_mouse-gap-43-mab-cl10847-azide-and-bsa-free-282202512135814.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL10847
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P17677, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for GAP-43 Antibody (CL10847) - Azide and BSA Free
![GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]](https://resources.bio-techne.com/images/products/nbp3-44282_mouse-gap-43-mab-cl10847-azide-and-bsa-free-282202512154127.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]")
Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]
Staining of human cerebellum shows moderate positivity in neuropil.![GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]](https://resources.bio-techne.com/images/products/nbp3-44282_mouse-gap-43-mab-cl10847-azide-and-bsa-free-282202512394437.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]")
Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]
Staining of human cerebral cortex shows strong positivity in neuronal processes in neuropil.Applications for GAP-43 Antibody (CL10847) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GAP-43
Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 Antibody (CL10847) - Azide and BSA Free
Product Specific Notices for GAP-43 Antibody (CL10847) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...