GAP-43 Antibody (CL10855)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21182

Novus Biologicals, part of Bio-Techne
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NBP3-21182-100ul
NBP3-21182-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10855

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAP-43 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Synthetic Peptide of P17677. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAP-43 Antibody (CL10855)

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-21182] -

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-21182] -

Staining of human cerebellum shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-21182] -

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-21182] -

Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-21182] -

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-21182] -

Staining of human cerebral cortex shows strong positivity in neuronal processes in the neuropil.
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Applications for GAP-43 Antibody (CL10855)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody (CL10855)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GAP-43 Antibody (CL10855)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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