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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Equine, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH.

Reactivity Notes

Reactivity to Canine, Chicken, and Primate reported in scientific literature (PMID: 30647968, 30819546, and 24249398 respectively). Drosophila reactivity reported in scientific literature (PMID: 30819546).

Localization

Neuronal Marker, developing and regenerating axons and growth cone.

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for GAP-43 Antibody [mFluor Violet 450 SE]

GAP-43 Antibody [mFluor Violet 450 SE] [NB300-143MFV450] -

GAP-43 Antibody [mFluor Violet 450 SE] [NB300-143MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

Applications for GAP-43 Antibody [mFluor Violet 450 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GAP-43

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody [mFluor Violet 450 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAP-43 Antibody [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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