GAP-43 [p Ser41] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-179
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Finch (100%), Human (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH. Accession # P07936
Modification
p Ser41
Localization
Growth cone
Specificity
Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Marker
Neuronal Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GAP-43 [p Ser41] Antibody
![Western Blot: GAP-43 [p Ser41] Antibody [NB300-179]](https://resources.bio-techne.com/images/products/GAP43-[p-Ser41]-Antibody-Western-Blot-NB300-179-img0002.jpg "Western Blot: GAP-43 [p Ser41] Antibody [NB300-179]")
Western Blot: GAP-43 [p Ser41] Antibody [NB300-179]
Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.Applications for GAP-43 [p Ser41] Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
NB300-179 can be used in Western blot where a band is seen at ~ 43 kDa, representing phosphorylated Gap43. In some tissues the antibody also recognizes a lower MW band that is also recognized by the PAN Gap43 antibody and, thus, it is likely to be a breakdown product. Immunohistochemical analysis has not yet been determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GAP-43
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Entrez Gene IDs
14432 (Mouse)
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 [p Ser41] Antibody
Product Specific Notices for GAP-43 [p Ser41] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...