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GAP-43 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41123

Novus Biologicals, part of Bio-Techne
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NBP1-41123
NBP1-41123SS

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline, Hamster, Primate

Cited:

Human, Mouse, Rat, Hamster

Applications

Validated:

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

GAP-43 from rat brain

Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAP-43 Antibody

Immunohistochemistry: GAP-43 Antibody [NBP1-41123]

Immunohistochemistry: GAP-43 Antibody [NBP1-41123]

Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP1-41123]

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP1-41123]

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-41123] - Staining of a mature retinal ganglion cell demonstrating axon outgrowth.
Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123] - Longitudinal [11C]GV1-57 imaging monitors mature OSN regeneration. Immunoblotting of immature neurons (GAP-43), mature neurons (OMP), and sustentacular cells (REEP6) in septal OE tissue collected from animals 2 or 11 weeks after ZnSO4 or vehicle treatment. Red arrows indicate DVR values and the corresponding immunoblots of OE tissue collected from the same individual animals 11 weeks after treatment. Purple arrows indicate DVR values representative of [11C]GV1-57 imaging 2 weeks after treatment and corresponding immunoblots of OE tissue collected from a separate cohort of animals. n = 2 per group at 2 weeks after treatment; n = 1 per group at 11 weeks after treatment. Image collected and cropped by CiteAb from the following publication (https://www.jci.org/articles/view/89162), licensed under a CC-BY license.

Applications for GAP-43 Antibody

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 28112682)

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry

1:400-1:1000

Immunohistochemistry-Frozen

1:400-1:1000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24686445)

Western Blot

1:1000
Application Notes
In Western blot, a band can be seen at 43-48 kDa.

Reviewed Applications

Read 1 review rated 4 using NBP1-41123 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Entrez Gene IDs

14432 (Mouse)

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAP-43 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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