GAP-43 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54668

Novus Biologicals, part of Bio-Techne
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NBP2-54668
NBP2-54668-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAP-43 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: KEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAP-43 Antibody

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP2-54668]

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP2-54668]

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-54668] - Staining of human cell line SH-SY5Y shows localization to plasma membrane.
Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668] - Staining in human cerebral cortex and pancreas tissues using anti-GAP43 antibody. Corresponding GAP43 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668] - Staining of human cerebral cortex shows high expression.
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Applications for GAP-43 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAP-43 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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