GAPDH-2 Antibody (2E3-2E10)
Novus Biologicals, part of Bio-Techne | Catalog # H00026330-M01
Key Product Details
Species Reactivity
Human, E. coli
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E3-2E10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GAPDS (AAH36373, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
Reactivity Notes
Human. Other species not tested.
Specificity
GAPDHS - glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for GAPDH-2 Antibody (2E3-2E10)
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]](https://resources.bio-techne.com/images/products/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]")
Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]
Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]](https://resources.bio-techne.com/images/products/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg "Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]")
Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]
Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.
Applications for GAPDH-2 Antibody (2E3-2E10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GAPDH-2
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase 2
Alternate Names
GAPD2, GAPDH2, GAPDHS, HSD-35
Entrez Gene IDs
26330 (Human)
Gene Symbol
GAPDHS
OMIM
609169 (Human)
UniProt
Additional GAPDH-2 Products
Product Documents for GAPDH-2 Antibody (2E3-2E10)
Product Specific Notices for GAPDH-2 Antibody (2E3-2E10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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