GAPDH Antibody (6C5cc) [DyLight 680]

Western Blot: GAPDH Antibody (6C5cc) [DyLight 680] [NB600-502FR] -

Western Blot: GAPDH Antibody (6C5cc) [DyLight 680] [NB600-502FR] - Prolonged post-ischemic stroke inflammation in EcoHIV-infected brains. Mice were infected with EcoHIV & subjected to stroke as in Fig. 1. mRNA expression of cytokines IL1 beta (a) & TNF alpha (b) chemokines CXCL1 (c) & CCL2 (d), & cellular activation markers GFAP (e) & Iba1 (f) were assessed by real-time qPCR 7 days post stroke. Sham, n = 6; Stroke, n = 12 mice per group, 3 independent experiments. Time course of GFAP (g) & Iba1 (h) protein expression levels as quantified by western blotting in sham & ischemic stroke animals at days 1, 4, 7, & 14 post-stroke in mock (M) & EcoHIV-infected (E) mice. Representative blots are shown, & quantified results are illustrated on the bar graphs. n = 5–12 mice per group, 3 independent experiments. Whiskers-box plots represent centerline median, with interquartile range & min-max whiskers. Source data are provided as a Source Data file. *p < 0.05; one-way ANOVA, followed by Tukey multiple comparison test (a–f) & unpaired t test (g, h) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043599), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody (6C5cc) [DyLight 680] [NB600-502FR] -

Western Blot: GAPDH Antibody (6C5cc) [DyLight 680] [NB600-502FR] - Effective targeting of EcoHIV diminishes post-ischemic stroke inflammation. Mice were infected, treated with ART-7 & ART-11, & subjected to ischemic stroke as in Fig. 6b, c. mRNA levels of cellular activation markers Iba1 (a) & GFAP (b) 7 days post stroke. Protein expression levels of Iba1 (c), GFAP (d), & ICAM1 (e) were quantified by immunoblotting in sham & ischemic stroke animals at day 14 post-stroke in mock (M) & EcoHIV-infected (E) mice that were treated with ART-7 (E + 7) or ART-11 (E + 11). Representative blots are shown, & quantified results are illustrated on the bar graphs. mRNA levels of anti-inflammatory markers ICAM-5 (f) & FoxP3 (g), & tissue degrading enzymes MMP2 (h) & MMP9 (i) were quantified by RT-qPCR. Impact of therapy on viral DNA genome levels was evaluated in ipsilateral hemisphere (j) & spleen (k); n = 5–16 mice per group, 2 independent experiments. Whiskers-box plots represent centerline median, with interquartile range & min-max whiskers. Other graphs represent data as mean & SEM with individual data points. Source data are provided as a Source Data file. *p < 0.05 or **p < 0.01; one-way ANOVA, followed by Tukey multiple comparison test (a, b & f–k), & unpaired t test (c–e) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043599), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody (6C5cc) [DyLight 680] [NB600-502FR] -

Western Blot: GAPDH Antibody (6C5cc) [DyLight 680] [NB600-502FR] - EcoHIV diminishes post-ischemic stroke NVU recovery. Mice were infected with EcoHIV & subjected to stroke as in Fig. 1. Brain sections were stained for laminin (a), ICAM-1 (b) & P-selectin (c) 24 h post stroke, & quantified for mean fluorescence index (MFI); n = 6 mice per group, 10 microvessels per mice, 2 independent experiments. d Time course of ICAM1 expression levels as quantified by western blotting in sham & ischemic stroke animals at days 1, 4, 7, & 14 post-stroke both in mock (M) & EcoHIV-infected (E) mice. Representative blots are shown, & quantified results from 5–12 samples per group are illustrated on the bar graphs. e Representative image (left) & quantified results (right) of infiltration of the infarct area by Lys6g immunoreactive cells (neutrophils) at 24 h post-ischemic stroke. The sections were also stained for MAP2 (neurons) & Hoechst (nuclei). Absence of MAP2 staining indicates infarct area. Data quantified from 6 mice per group, 2 independent experiments, 4 fields of view per mice at ×20 magnification; Z stack images. Whiskers-box plots represent centerline median, with interquartile range & min-max whiskers. Source data are provided as a Source Data file. **p < 0.01; ***p < 0.001; unpaired t test. a–c Scale bars: 40 µm; e scale bar: 320 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043599), licensed under a CC-BY license. Not internally tested by Novus Biologicals.