GAPDH Antibody (BLR103H)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14695
Recombinant Monoclonal
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR103H
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Immunogen was a peptide representing a region between residue 225 and 275 of human glyceraldehyde-3-phosphate dehydrogenase using the numbering given in entry NP_002037.2 (Gene ID 2597).
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for GAPDH Antibody (BLR103H)
Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695]
Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.Immunohistochemistry: GAPDH Antibody (BLR103H) [NBP3-14695]
Immunohistochemistry: GAPDH Antibody (BLR103H) [NBP3-14695] - Rabbit anti-GAPDH recombinant monoclonal antibody. Secondary HRP-conjugated goat anti-rabbit IgG. Substrate:DABWestern Blot: GAPDH Antibody (BLR103H) [NBP3-14695]
Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (50 ug) from HeLa, Jurkat, RKO, U2OS, GaMG, LNCaP, MCF-7, K-562, HEK293T, and Hep-G2 cells prepared using NETN lysis buffer. Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second.Applications for GAPDH Antibody (BLR103H)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunohistochemistry-Paraffin
1:100 to 1:500
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Gene Symbol
GAPDH
Additional GAPDH Products
Product Documents for GAPDH Antibody (BLR103H)
Product Specific Notices for GAPDH Antibody (BLR103H)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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