GAPDH Antibody (RM114)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77402
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Goat, Monkey
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM114
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GAPDH antibody developed against the C-terminus of GAPDH
Reactivity Notes
RM114 shows strong reactivity to Human and Monkey GAPDH. RM114 shows weaker reactivity to Mouse, Goat GAPDH and may require higher antibody dilution usage.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GAPDH Antibody (RM114)
![Western Blot: GAPDH Antibody (RM114) [NBP2-77402]](https://resources.bio-techne.com/images/products/GAPDH-Antibody-RM114-Western-Blot-NBP2-77402-img0002.jpg "Western Blot: GAPDH Antibody (RM114) [NBP2-77402]")
Western Blot: GAPDH Antibody (RM114) [NBP2-77402]
Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).![Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402]](https://resources.bio-techne.com/images/products/GAPDH-Antibody-RM114-Immunocytochemistry-Immunofluorescence-NBP2-77402-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402]")
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402]
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402] - Immunocytochemical staining of HeLa cells, using NBP2-77402 (red). Nuclei have been labeled with DAPI (blue).Applications for GAPDH Antibody (RM114)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:200
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:200
Immunoprecipitation
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Gene Symbol
GAPDH
Additional GAPDH Products
Product Documents for GAPDH Antibody (RM114)
Product Specific Notices for GAPDH Antibody (RM114)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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