GART Antibody (4D6-1D5)

Western Blot: GART Antibody (4D6-1D5) [H00002618-M01]

Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - GART monoclonal antibody (M01), clone 4D6-1D5 Analysis of GART expression in Hela S3 NE.

Western Blot: GART Antibody (4D6-1D5) [H00002618-M01]

Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.Lane 1: GART transfected lysate(46 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: GART Antibody (4D6-1D5) [H00002618-M01]

Immunoprecipitation: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GART MaxPab rabbit polyclonal antibody.

Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] -

Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - The role of HIF-1 in purinosome formation.a, quantifying the number of purinosome-containing cells in normoxia or hypoxia (24 h) in purine-rich medium & normoxia in purine-depleted medium (purine -ve), cells in hypoxia transfected with siRNA to HIF-1 alpha (+ siRNA), & cells in purine-rich medium supplemented with DFX. Data shown are n = 3, mean ± S.E., total number of cells counted are shown in parentheses. b, time course of purinosome formation in hypoxia shows the number of purinosome-containing cells steadily increases after 3 h in hypoxia. Re-oxygenation of the samples after hypoxic incubation for 10 h reverts the number purinosome-containing cells back to normoxic levels. Data shown is n = 3, mean ± S.E., total number of cells counted are shown in parentheses. c, time course of HIF-1 alpha stabilization in hypoxia shows maximum HIF-1 alpha protein expression levels at 3 h in hypoxia, after which the HIF-1 alpha expression decreases. The positions of molecular markers are shown for each blot; uncropped blots with overlaid markers are deposited in the raw data files. d, the effect of hypoxia on the transcription of purine biosynthesis enzymes measured by qPCR. Vascular endothelial growth factor (VEGF) & HIF-1 alpha are controls. Data shown are n = 5, mean ± S.E. e, the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, & no significant increase in the purine enzymes was detected between normoxic (21% oxygen) & hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot; uncropped blots with overlaid markers are deposited in the raw data files. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32439803), licensed under a CC-BY license. Not internally tested by Novus Biologicals.