Gastric Lipase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60930

Novus Biologicals, part of Bio-Techne
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NB110-60930
NB110-60930SS
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.9 mg/ml

View all Gastric Lipase Antibodies »

Product Specifications

Immunogen

A synthetic peptide within residues 1-100 of human Gastric Lipase. [Swiss-Prot# P07098]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (87%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gastric Lipase Antibody - BSA Free

Western Blot: Gastric Lipase AntibodyBSA Free [NB110-60930]

Western Blot: Gastric Lipase AntibodyBSA Free [NB110-60930]

Western Blot: Gastric Lipase Antibody [NB110-60930] - Detection of LIPF in liver lysate using NB110-60930. Lane 1: human Lane 2: mouse

Applications for Gastric Lipase Antibody - BSA Free

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
This Gastric Lipase antibody is useful for Western blot analysis, where a band is seen at ~45 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gastric Lipase

Gastric lipase (GL/LIPF or gastric triacylglycerol lipase) is an extremophilic enzyme which retains its activity in gastric juices fully at pH 2 under the presence of pepsin in stomach wherein the gastrointestinal lipolysis of dietary fat is initiated. Gastric lipase belongs to AB hydrolase superfamily/lipase family and is a secreted protein which is not inhibited by bile salts in the gastrointestinal tract, whereas pancreatic lipase (main lipase involved in fat digestion) requires a cofactor, namely colipase, to counteract the inhibitory effects of bile salts on lipase adsorption at lipid-water interfaces. Due to its acid resistant properties, it has been proposed as a candidate for the treatment of pancreatic enzyme insufficiency observed in chronic pancreatitis and cystic fibrosis, the gastrointestinal disorders which are characterized by exocrine pancreatic secretion deficiency, gastric acid secretion not being neutralized by pancreatic bicarbonate, and pH of the small intestine contents reaching as low as 2-3.

Long Name

Gastric triacylglycerol lipase

Alternate Names

GL, LIPF

Gene Symbol

LIPF

Additional Gastric Lipase Products

Product Documents for Gastric Lipase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Gastric Lipase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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