Gastrin Antibody (PD00-37)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32370

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32370
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-37

Concentration

1 mg/ml

View all Gastrin I Antibodies »

Product Summary for Gastrin Antibody (PD00-37)

Immunogen

Synthetic peptide within Human Gastrin (internal sequence). (Uniprot: P01350)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gastrin Antibody (PD00-37)

Gastrin Antibody (PD00-37)

Immunohistochemistry: Gastrin Antibody (PD00-37) [NBP3-32370]

Immunohistochemistry: Gastrin Antibody (PD00-37) [NBP3-32370] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Gastrin antibody (NBP3-32370) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Gastrin Antibody (PD00-37)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gastrin I

Gastrin, which is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, is a hormone whose main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Gastrin also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Gastrin is regulated by epidermal growth factor in both mice and humans. Gastrin is excreted in excess by pancreatic tumors in the Zollinger- Ellison syndrome. Gastrin maps to human chromosome 17q-21. Gastrin- Releasing Peptide (GRP) stimulates the release of gastrin as well as other gastrointestinal hormones, in addition to acting as an autocrine growth factor for certain cell types. High levels of GRP are found in the human lung just after birth and levels decrease thereafter in parallel with the observed disease in a number of pulmonary neuroendocrine cells. GRP is known to promote lung tumorigenesis in model systems and, interestingly, is induced by retinoic acid. GRP is involved in several functions with the hypothalamus, and is thought to play a role in regulating pituitary hormone secretion. GRP maps to human chromosome 18q21.

Alternate Names

G17, GAST, Gastrin-17

Gene Symbol

GAST

Additional Gastrin I Products

Product Documents for Gastrin Antibody (PD00-37)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Gastrin Antibody (PD00-37)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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