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Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03282

Novus Biologicals, part of Bio-Techne
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NBP3-03282-100ul
NBP3-03282-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human Gastrin-releasing Peptide/Bombesin/Neuromedin C (NP_002082.2). MRGRELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSEDSSNFKDVGSKGKVGRLSAPGSQREGRNPQLNQQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free

Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C AntibodyBSA Free [NBP3-03282]

Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C AntibodyBSA Free [NBP3-03282]

Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [NBP3-03282]

Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [NBP3-03282]

Immunocytochemistry/Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Immunofluorescence analysis of HeLa cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [NBP3-03282]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [NBP3-03282]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gastrin-releasing Peptide/Bombesin

Gastrin-releasing peptide receptor (GRPR) is a Bombesin Receptor. In addition to its involvement in autism and mental retardation, GRPR may also play a role in the progression of cancer. Nicotine exposure induces an increase in GRPR expression in the lung Shriver et al. (2000). GRPR has been reported in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach, and uterus. It has also been shown to be expressed in several cancers, including those in breast, colon, lung, ovary, pancreas, and prostate.

Alternate Names

Bombesin, Gastrinreleasing Peptide, GRP, GRP10

Gene Symbol

GRP

Additional Gastrin-releasing Peptide/Bombesin Products

Product Documents for Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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