GAT-1/SLC6A1 Antibody (JE34-61)

Western Blot: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372]

Western Blot: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372] - Western blot analysis of GAT-1/SLC6A1 on different lysates with Rabbit anti-GAT-1/SLC6A1 antibody (NBP3-32372) at 1/1,000 dilution.

Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse cerebellum tissue lysate (70 heat)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse brain tissue lysate (70 heat)
Lane 5: Rat brain tissue lysate
Lane 6: Rat brain tissue lysate (70 heat)

Lysates/proteins at 30 ug/Lane.

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32372) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372]

Immunohistochemistry: GAT-1/SLC6A1 Antibody (JE34-61) [NBP3-32372] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GAT-1/SLC6A1 antibody (NBP3-32372) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32372) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.