GAT-1/SLC6A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61097

Novus Biologicals, part of Bio-Techne
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NB100-61097
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GAT-1/SLC6A1 Antibodies »

Product Summary for GAT-1/SLC6A1 Antibody

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P23978

Reactivity Notes

It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~67 kDa GAT-1/SLC6A1 protein. Immunolabeling is blocked by pre-adsorption of antibody with the peptide used to generate the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAT-1/SLC6A1 Antibody

Western Blot: GAT-1/SLC6A1 Antibody [NB100-61097]

Western Blot: GAT-1/SLC6A1 Antibody [NB100-61097]

Western Blot: GAT-1/SLC6A1 Antibody [NB100-61097] - Rat hippocampal homogenate showing specific immunolabeling of the~ 67k GAT-1 protein.

Applications for GAT-1/SLC6A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAT-1/SLC6A1

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Long Name

GABA Transporter 1

Alternate Names

GABATHG, GABATR, GAT1, SLC6A1

Entrez Gene IDs

50676 (Rat)

Gene Symbol

SLC6A1

UniProt

P23978 (Rat)

Additional GAT-1/SLC6A1 Products

Product Documents for GAT-1/SLC6A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAT-1/SLC6A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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