GAT-1/SLC6A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13166

Novus Biologicals, part of Bio-Techne
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NBP3-13166
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (94%), Human (98%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAT-1/SLC6A1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse GAT-1/SLC6A1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GAT-1/SLC6A1 Antibody

Western Blot: GAT-1/SLC6A1 Antibody [NBP3-13166]

Western Blot: GAT-1/SLC6A1 Antibody [NBP3-13166]

Western Blot: GAT-1/SLC6A1 Antibody [NBP3-13166] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: GAT-1/SLC6A1 Antibody [NBP3-13166]

Immunocytochemistry/ Immunofluorescence: GAT-1/SLC6A1 Antibody [NBP3-13166]

Immunocytochemistry/Immunofluorescence: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: GAT-1/SLC6A1 Antibody [NBP3-13166]

Immunohistochemistry-Frozen: GAT-1/SLC6A1 Antibody [NBP3-13166]

Immunohistochemistry-Frozen: GAT-1/SLC6A1 Antibody [NBP3-13166] - GAT-1/SLC6A1 antibody detects GAT-1/SLC6A1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse hippocampus. Green: GAT-1/SLC6A1 protein stained by GAT-1/SLC6A1 antibody (NBP3-13166) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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Applications for GAT-1/SLC6A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAT-1/SLC6A1

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Long Name

GABA Transporter 1

Alternate Names

GABATHG, GABATR, GAT1, SLC6A1

Gene Symbol

SLC6A1

Additional GAT-1/SLC6A1 Products

Product Documents for GAT-1/SLC6A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAT-1/SLC6A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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