GATA-2 Antibody (1G5)

Novus Biologicals, part of Bio-Techne | Catalog # H00002624-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GATA-2 Antibodies »

Product Specifications

Immunogen

GATA2 (AAH18988, 1 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGK

Localization

Nuclear

Specificity

GATA2 - GATA binding protein 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GATA-2 Antibody (1G5)

Western Blot: GATA-2 Antibody (1G5) [H00002624-M02]

Western Blot: GATA-2 Antibody (1G5) [H00002624-M02]

Western Blot: GATA-2 Antibody (1G5) [H00002624-M02] - GATA2 monoclonal antibody (M02), clone 1G5 Analysis of GATA2 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: GATA-2 Antibody (1G5) [H00002624-M02]

Immunocytochemistry/ Immunofluorescence: GATA-2 Antibody (1G5) [H00002624-M02]

Immunocytochemistry/Immunofluorescence: GATA-2 Antibody (1G5) [H00002624-M02] - Analysis of monoclonal antibody to GATA2 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: GATA-2 Antibody (1G5) [H00002624-M02] - Detection limit for recombinant GST tagged GATA2 is approximately 0.03ng/ml as a capture antibody.

Applications for GATA-2 Antibody (1G5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GATA-2

The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells (Tsai et al., 1994 [PubMed 8078582]). GATA1 (MIM 305371) is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation (Briegel et al., 1993 [PubMed 8504932]). GATA3 (MIM 131320) expression is restricted to T-lymphoid cells and some nonhematopoietic cell types, including embryonic stem cells.[supplied by OMIM]

Alternate Names

GATA2

Entrez Gene IDs

2624 (Human)

Gene Symbol

GATA2

OMIM

137295 (Human)

UniProt

AAH18988 (Human)

Additional GATA-2 Products

Product Documents for GATA-2 Antibody (1G5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-2 Antibody (1G5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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