GATA-2 Antibody (1K8H6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16548

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16548-100ul
NBP3-16548-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1K8H6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GATA-2 Antibodies »

Product Summary for GATA-2 Antibody (1K8H6)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GATA-2 (NP_116027.2). MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSVYPGAGGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GATA-2 Antibody (1K8H6)

Western Blot: GATA-2 Antibody (1K8H6) [NBP3-16548]

Western Blot: GATA-2 Antibody (1K8H6) [NBP3-16548]

Western Blot: GATA-2 Antibody (1K8H6) [NBP3-16548] - Western blot analysis of extracts of various cell lines, using GATA-2 Rabbit mAb (NBP3-16548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Knockout Validated: GATA-2 Antibody (1K8H6) [NBP3-16548]

Knockout Validated: GATA-2 Antibody (1K8H6) [NBP3-16548]

Knockout Validated: GATA-2 Antibody (1K8H6) [NBP3-16548] - Western blot analysis of extracts from normal (control) and GATA-2 knockout (KO) HeLa cells, using GATA-2 antibody (NBP3-16548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for GATA-2 Antibody (1K8H6)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-2

The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells (Tsai et al., 1994 [PubMed 8078582]). GATA1 (MIM 305371) is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation (Briegel et al., 1993 [PubMed 8504932]). GATA3 (MIM 131320) expression is restricted to T-lymphoid cells and some nonhematopoietic cell types, including embryonic stem cells.[supplied by OMIM]

Alternate Names

GATA2

Gene Symbol

GATA2

Additional GATA-2 Products

Product Documents for GATA-2 Antibody (1K8H6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GATA-2 Antibody (1K8H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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