GATA-3 Antibody (OTI8H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45810

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8H4

Concentration

1 mg/ml

View all GATA-3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-3 Antibody (OTI8H4)

Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis (35 ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody at 1:200.
Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Mouse lung tissue section stained with GATA-3 antibody. IHC-P image submitted by a verified customer review.
Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Flow cytometric Analysis of penetrated MCF-7 cells, compared to a nonspecific negative control antibody, (Blue). The right is HUVEC cells as negtive control (1:100).
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Applications for GATA-3 Antibody (OTI8H4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:200

Western Blot

1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-45810 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (OTI8H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-3 Antibody (OTI8H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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