GATA-4 Antibody (6H10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47540

Novus Biologicals, part of Bio-Techne
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NBP1-47540
NBP1-47540SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 6H10

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all GATA-4 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GATA4 expressed in E. coli. [UniProt# P43694]

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-4 Antibody (6H10)

Western Blot: GATA-4 Antibody (6H10) [NBP1-47540]

Western Blot: GATA-4 Antibody (6H10) [NBP1-47540]

Western Blot: GATA4 Antibody (6H10) [NBP1-47540] - Western blot analysis using anti-GATA4 mAb against rat fetal heart (1) and adult heart (2) tissues lysate.

Applications for GATA-4 Antibody (6H10)

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500-1:2000
Application Notes
This GATA4 (6H10) antibody is useful for Western blot and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-4

GATA4, also known as GATA binding protein 4, is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

Alternate Names

GATA4

Entrez Gene IDs

2626 (Human); 14463 (Mouse)

Gene Symbol

GATA4

UniProt

P43694

Additional GATA-4 Products

Product Documents for GATA-4 Antibody (6H10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GATA-4 Antibody (6H10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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