GATA-4 Antibody (OTI9F9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47765

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI9F9

Concentration

0.17 mg/ml

View all GATA-4 Antibodies »

Product Summary for GATA-4 Antibody (OTI9F9)

Immunogen

Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens GATA binding protein 4 (GATA4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-4 Antibody (OTI9F9)

Western Blot: GATA-4 Antibody (OTI9F9) [NBP1-47765]

Western Blot: GATA-4 Antibody (OTI9F9) [NBP1-47765]

Western Blot: GATA-4 Antibody (OTI9F9) [NBP1-47765] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GATA4 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody (OTI9F9) [NBP1-47765]

Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody (OTI9F9) [NBP1-47765]

Immunocytochemistry/Immunofluorescence: GATA-4 Antibody (OTI9F9) [NBP1-47765] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4.
Immunohistochemistry-Paraffin: GATA-4 Antibody (OTI9F9) [NBP1-47765]

Immunohistochemistry-Paraffin: GATA-4 Antibody (OTI9F9) [NBP1-47765]

Immunohistochemistry-Paraffin: GATA-4 Antibody (OTI9F9) [NBP1-47765] - Staining of paraffin-embedded Human endometrium tissue using anti-GATA4 mouse monoclonal antibody.
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Applications for GATA-4 Antibody (OTI9F9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.17 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-4

GATA4 encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects

Alternate Names

GATA4

Entrez Gene IDs

2626 (Human)

Gene Symbol

GATA4

Additional GATA-4 Products

Product Documents for GATA-4 Antibody (OTI9F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-4 Antibody (OTI9F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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