GATM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89211

Novus Biologicals, part of Bio-Techne
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NBP1-89211
NBP1-89211-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GATM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGRTLTGWVQRTFQSTQAATASSRNSCAADDKATEPLPKDCPVSSYNEWDPLEEVIVGRAENACVPPFTIEVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNILKTEGVTVRRPDPIDWSLKYKTPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GATM Antibody

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211] - Staining in human kidney and skeletal muscle tissues . Corresponding GATM RNA-seq data are presented for the same tissues.
Western Blot: GATM Antibody [NBP1-89211]

Western Blot: GATM Antibody [NBP1-89211]

Western Blot: GATM Antibody [NBP1-89211] - Analysis in human kidney tissue.
Immunocytochemistry/ Immunofluorescence: GATM Antibody [NBP1-89211]

Immunocytochemistry/ Immunofluorescence: GATM Antibody [NBP1-89211]

Immunocytochemistry/Immunofluorescence: GATM Antibody [NBP1-89211] - Staining of human cell line RH-30 shows localization to mitochondria. Antibody staining is shown in green.
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Applications for GATM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATM

GATM encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders.

Alternate Names

AGATtransamidinase, AT, EC 2.1.4, EC 2.1.4.1, glycine amidinotransferase (L-arginine:glycine amidinotransferase), glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, Transamidinase

Gene Symbol

GATM

Additional GATM Products

Product Documents for GATM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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