GCAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68721

Novus Biologicals, part of Bio-Techne
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NBP2-68721
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Primate - Macaca mulatta (Rhesus Macaque)

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCAP2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GQEFSWEEAEAAGEIDVAELQEWYKKFVMECPSGTLFMHEFKRFFKVTDDEEASQYVEGMFRAFDKNG

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) .

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCAP2 Antibody

Western Blot: GCAP2 Antibody [NBP2-68721]

Western Blot: GCAP2 Antibody [NBP2-68721]

Western Blot: GCAP2 Antibody [NBP2-68721] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: GCAP2 Antibody [NBP2-68721]

Immunohistochemistry-Paraffin: GCAP2 Antibody [NBP2-68721]

Immunohistochemistry-Paraffin: GCAP2 Antibody [NBP2-68721] - Staining of human retina shows strong cytoplasmic positivity in cones and outer plexiform layer.

Applications for GCAP2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Frozen

Reported in scientific literature (PMID:35024589)

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
Recommended conditions for IHC,Retrieval method: HIER pH6.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCAP2

Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP 1 and GCAP 2. It has been demonstrated that both GCAP 1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 is predominantly localized in the photoreceptor outer segments, while GCAP 2 is found in retina samples.

Alternate Names

DKFZp686E1183, GCAP 2, GCAP2guanylate cyclase-activating protein, photoreceptor 2, Guanylate cyclase activator 1B, guanylate cyclase activator 1B (retina), guanylyl cyclase-activating protein 2, GUCA2, RP48

Gene Symbol

GUCA1B

Additional GCAP2 Products

Product Documents for GCAP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GCAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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