GCC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83609

Novus Biologicals, part of Bio-Techne
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NBP1-83609
NBP1-83609-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDPADTSSSDSLTQALQLAAANEPTFFLYAEQLARKEVEITSLRKQKHRLEVEVHQLQDRLLEEGERHREEVAALQSHIEKNIRDQSREGANLEYLKNIIYRFLTLPDSLGRQQTLTAILTILHFSPEEKQVIMRLPT

Reactivity Notes

Canine reactivity reported by a customer review

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCC1 Antibody

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609]

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609]

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human colon, kidney, liver and testis using Anti-GCC1 antibody NBP1-83609 (A) shows similar protein distribution across tissues to independent antibody NBP1-83610 (B).
Western Blot: GCC1 Antibody [NBP1-83609]

Western Blot: GCC1 Antibody [NBP1-83609]

Western Blot: GCC1 Antibody [NBP1-83609] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: GCC1 Antibody [NBP1-83609]

Immunocytochemistry/ Immunofluorescence: GCC1 Antibody [NBP1-83609]

Immunocytochemistry/Immunofluorescence: GCC1 Antibody [NBP1-83609] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
View 5 more images

Applications for GCC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-83609 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCC1

GCC1 is encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport. [provided by RefSeq]

Alternate Names

FLJ22035, GCC1P, GCC88Golgi coiled-coil protein 1, golgi coiled-coil 1, GRIP and coiled-coil domain containing 1, GRIP and coiled-coil domain-containing protein 1, MGC20706, peripheral membrane Golgi protein

Entrez Gene IDs

79571 (Human)

Gene Symbol

GCC1

UniProt

Q96CN9 (Human)

Additional GCC1 Products

Product Documents for GCC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GCC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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