GCKR Antibody (OTI1E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03396

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E7

Concentration

1 mg/ml

View all GCKR Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GCKR (NP_001417) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCKR Antibody (OTI1E7)

Western Blot: GCKR Antibody (OTI1E7) [NBP2-03396]

Western Blot: GCKR Antibody (OTI1E7) [NBP2-03396]

Western Blot: GCKR Antibody (1E7) [NBP2-03396] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-GCKR monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunocytochemistry/Immunofluorescence: GCKR Antibody (1E7) [NBP2-03396] Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCKR.
Immunohistochemistry-Paraffin: GCKR Antibody (OTI1E7) [NBP2-03396]

Immunohistochemistry-Paraffin: GCKR Antibody (OTI1E7) [NBP2-03396]

Immunohistochemistry-Paraffin: GCKR Antibody (1E7) [NBP2-03396] - Staining of paraffin-embedded Human Kidney tissue using anti-GCKR mouse monoclonal antibody.
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Applications for GCKR Antibody (OTI1E7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCKR

This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq].

Alternate Names

FGQTL5, GKRP, glucokinase (hexokinase 4) regulator, glucokinase (hexokinase 4) regulatory protein, Glucokinase regulator, glucokinase regulatory protein

Entrez Gene IDs

2646 (Human)

Gene Symbol

GCKR

Additional GCKR Products

Product Documents for GCKR Antibody (OTI1E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GCKR Antibody (OTI1E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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