GCN2 Antibody (1G2Z4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16112
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G2Z4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for GCN2 Antibody (1G2Z4)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCN2 (NP_001013725.2). MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNAL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GCN2 Antibody (1G2Z4)
Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] -
Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] -Analysis of various lysates, using GCN2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] -
Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] - Analysis of Rat brain, using GCN2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IHC-P-GCN2 Antibody (6E2X0) [NBP3-16112] -
IHC-P-GCN2 Antibody (6E2X0) [NBP3-16112] -Analysis of paraffin-embedded mouse kidney using GCN2 Rabbit mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for GCN2 Antibody (1G2Z4)
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GCN2
Long Name
General Control Nonrepressed 2-like Protein
Alternate Names
EIF2AK4
Gene Symbol
EIF2AK4
Additional GCN2 Products
Product Documents for GCN2 Antibody (1G2Z4)
Product Specific Notices for GCN2 Antibody (1G2Z4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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