GCN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38126

Novus Biologicals, part of Bio-Techne
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NBP2-38126
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCN2 Antibodies »

Product Summary for GCN2 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCN2 Antibody

Western Blot: GCN2 Antibody [NBP2-38126]

Western Blot: GCN2 Antibody [NBP2-38126]

Western Blot: GCN2 Antibody [NBP2-38126] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: GCN2 Antibody [NBP2-38126]

Immunocytochemistry/ Immunofluorescence: GCN2 Antibody [NBP2-38126]

Immunocytochemistry/Immunofluorescence: GCN2 Antibody [NBP2-38126] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126]

Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126]

Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126] - Staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.

Applications for GCN2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCN2

GCN2 is a sensor of amino acid deprivation. GCN2 was recently found to regulate lipid metabolism during leucine deprivation in addition to regulating amino acid metabolism.

Long Name

General Control Nonrepressed 2-like Protein

Alternate Names

EIF2AK4

Gene Symbol

EIF2AK4

UniProt

Q9P2K8

Additional GCN2 Products

Product Documents for GCN2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GCN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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