GEFT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14309

Novus Biologicals, part of Bio-Techne
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NBP2-14309
NBP2-14309-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GEFT Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: HSKHCLSVETEADSGQAGPYENWMLEPALATGEELPELTLLTTLLEGPGDKTQPPEEETLSQAPESEEEQKKKALERSMYV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GEFT Antibody

Western Blot: GEFT Antibody [NBP2-14309]

Western Blot: GEFT Antibody [NBP2-14309]

Western Blot: GEFT Antibody [NBP2-14309] - Analysis in control (vector only transfected HEK293T lysate) and ARHGEF25 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GEFT Antibody [NBP2-14309]

Immunohistochemistry-Paraffin: GEFT Antibody [NBP2-14309]

Immunohistochemistry-Paraffin: GEFT Antibody [NBP2-14309] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for GEFT Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GEFT

The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange (Souchet et al., 2002 [PubMed 11861769]).[supplied by OMIM]. Transcript Variant: This variant (1) represents the longer transcript (1).

Alternate Names

GEFTRAC/CDC42 exchange factor, Guanine nucleotide exchange factor GEFT, p63RhoGEFrho guanine nucleotide exchange factor 25, Rac/Cdc42/Rho exchange factor GEFT, Rho guanine nucleotide exchange factor (GEF) 25, RhoA/RAC/CDC42 exchange factor, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT

Gene Symbol

ARHGEF25

Additional GEFT Products

Product Documents for GEFT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GEFT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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