Gelsolin/GSN Antibody (GS-2C4)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-10067
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Bovine, Rabbit, Rat (Negative)
Cited:
Human
Applications
Validated:
ELISA, Immunoaffinity Purification, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Microarray, Western Blot
Cited:
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GS-2C4
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Full length native protein (purified) (Human).
Epitope
The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.
Reactivity Notes
Cross-reacts with Human, Cow, Pig and Rabbit. Does not react with Rat.
Localization
Cytoplasmic and Secreted
Specificity
The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Gelsolin/GSN Antibody (GS-2C4)
Western Blot: Gelsolin/GSN Antibody (GS-2C4) [NB110-10067]
Western Blot: Gelsolin/GSN Antibody (GS-2C4) [NB110-10067] - Human plasma, barium citrate ppt. was separated on SDS-PAGE and probed with Monoclonal AB To Gelsolin (MO) CL:GS-2C4. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Antibody dilution 1:2000.Applications for Gelsolin/GSN Antibody (GS-2C4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:1000
Application Notes
An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts. WB: Predicted molecular weight: 86 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 27121327).
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.9% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Gelsolin/GSN
Additional Gelsolin/GSN Products
Product Documents for Gelsolin/GSN Antibody (GS-2C4)
Product Specific Notices for Gelsolin/GSN Antibody (GS-2C4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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