Gelsolin/GSN Antibody (GT1656)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13559

Novus Biologicals, part of Bio-Techne
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NBP3-13559
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1656

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Gelsolin/GSN Antibodies »

Product Summary for Gelsolin/GSN Antibody (GT1656)

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gelsolin/GSN. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Gelsolin/GSN Antibody (GT1656)

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:500.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Gelsolin/GSN antibody [GT1656] detects Gelsolin/GSN protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000.
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Applications for Gelsolin/GSN Antibody (GT1656)

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (GT1656)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Gelsolin/GSN Antibody (GT1656)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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