Gemin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1801

Novus Biologicals, part of Bio-Techne
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NB100-1801
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Gemin 3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human DEAD (Asp-Glu-Ala-Asp) Box 20 using the numbering given in entry NP_009135.3 (GeneID 11218).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gemin 3 Antibody

Western Blot: Gemin 3 Antibody [NB100-1801]

Western Blot: Gemin 3 Antibody [NB100-1801]

Western Blot: Gemin 3 Antibody [NB100-1801] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1801. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1802 and two.
Immunohistochemistry: Gemin 3 Antibody [NB100-1801]

Immunohistochemistry: Gemin 3 Antibody [NB100-1801]

Immunohistochemistry: Gemin 3 Antibody [NB100-1801] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX20 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Applications for Gemin 3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Gemin 3

DDX20 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. In addition to its role in snRNP assembly, DDX20 can function as a transcriptional repressor. DDX20 is also known as probable ATP-dependent RNA helicase DDX20, DEAD box protein 20, DEAD box protein DP 103, component of gems 3, gemin-3, DP103, and DKFZp434H052.

Alternate Names

Component of gems 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD, DKFZp434H052, DP103DEAD-box protein DP103, EC 3.6.4.13, Gemin-3, GEMIN3gemin-3, probable ATP-dependent RNA helicase DDX20, SMN-interacting protein

Entrez Gene IDs

11218 (Human)

Gene Symbol

DDX20

UniProt

NP_009135.3 (Human)

Additional Gemin 3 Products

Product Documents for Gemin 3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Gemin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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