Geminin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14058

Novus Biologicals, part of Bio-Techne
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NBP2-14058
NBP2-14058-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Geminin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: RRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQYMAELIERLNGEPLDN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Geminin Antibody

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-14058]

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-14058]

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-14058] - Staining in human testis and skeletal muscle tissues using anti-GMNN antibody. Corresponding GMNN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Geminin Antibody [NBP2-14058]

Immunocytochemistry/ Immunofluorescence: Geminin Antibody [NBP2-14058]

Immunocytochemistry/Immunofluorescence: Geminin Antibody [NBP2-14058] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-14058]

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-14058]

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-14058] - Staining of human skeletal muscle shows low expression as expected.
View 1 more images

Applications for Geminin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Geminin

Geminin is a multifunctional nuclear protein invloved in embyro development/differentiation, cell cycle regulation and DNA replciation. Originally defined as an inhibitor of DNA replication and substrate of the anaphase promoting complex, Geminin has also been shown to expand the neural plate in the developing embryos.

Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.

Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Gene Symbol

GMNN

Additional Geminin Products

Product Documents for Geminin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Geminin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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