Gephyrin/GPHN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16641

Novus Biologicals, part of Bio-Techne
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NBP2-16641
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (100%), Porcine (100%), Rhesus Macaque (100%), Xenopus (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Gephyrin/GPHN Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gephyrin/GPHN. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (99%).

Localization

Cell junction; synapse (By similarity); synapse; postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasm; cytoskeleton (By similarity);

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gephyrin/GPHN Antibody

Western Blot: Gephyrin/GPHN Antibody [NBP2-16641]

Western Blot: Gephyrin/GPHN Antibody [NBP2-16641]

Western Blot: Gephyrin/GPHN Antibody [NBP2-16641] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody [NBP2-16641]

Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody [NBP2-16641]

Immunocytochemistry/Immunofluorescence: Gephyrin/GPHN Antibody [NBP2-16641] - DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: Gephyrin/GPHN Antibody [NBP2-16641]

Immunohistochemistry-Frozen: Gephyrin/GPHN Antibody [NBP2-16641]

Immunohistochemistry-Frozen: Gephyrin/GPHN Antibody [NBP2-16641] - Frozen sectioned E13.5 Rat brain. Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.
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Applications for Gephyrin/GPHN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Gephyrin/GPHN

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq]

Alternate Names

GEPH, GPH, GPHN, GPHRYN

Gene Symbol

GPHN

UniProt

Q9NQX3 (Human)

Additional Gephyrin/GPHN Products

Product Documents for Gephyrin/GPHN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Gephyrin/GPHN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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