GFP Antibody (4B10B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22111

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4B10B2

Format

BSA Free

Concentration

1.0 mg/ml

View all GFP Antibodies »

Product Specifications

Immunogen

This GFP antibody was developed against purified recombinant fragment of GFP expressed in E. coli.

Reactivity Notes

Reacts with GFP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GFP Antibody (4B10B2) - BSA Free

Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]

Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.
Immunocytochemistry/ Immunofluorescence: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]

Immunocytochemistry/ Immunofluorescence: GFP Antibody (4B10B2) - BSA Free [NBP2-22111]

Immunocytochemistry/Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .
Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]

Western Blot: GFP Antibody (4B10B2)BSA Free [NBP2-22111]

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
View 2 more images

Applications for GFP Antibody (4B10B2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000. Use reported by customer review
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

Additional GFP Products

Product Documents for GFP Antibody (4B10B2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GFP Antibody (4B10B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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