GFP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56402

Novus Biologicals, part of Bio-Techne
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NB100-56402
NB100-56402SS
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GFP Antibodies »

Product Specifications

Immunogen

This GFP antibody was developed against amino acids 204-217 of Green Fluorescent Protein (GFP).

Reactivity Notes

This antibody reacts with Green Fluorescent Protein and is not species specific.

Specificity

The GFP antibody may recognize YFP (Yellow) and CFP (Cyan), as the amino acid sequence used as immunogen for this antibody is 100% homologous in YFP and CFP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GFP Antibody

Western Blot: GFP Antibody [NB100-56402]

Western Blot: GFP Antibody [NB100-56402]

Western Blot: GFP Antibody [NB100-56402] - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody (NB100-56402) at 1:1000 (0.5ug/ml).
Western Blot: GFP Antibody [NB100-56402]

Western Blot: GFP Antibody [NB100-56402]

Western Blot: GFP Antibody [NB100-56402] - Analysis of Green Fluorescent Protein in 20 ugs of A) transfected HeLa cells and B) untransfected HeLa cells using Green Fluorescent Protein antibody at 0.5 ug/ml.

Applications for GFP Antibody

Application
Recommended Usage

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

UniProt

P45974

Additional GFP Products

Product Documents for GFP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GFP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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