GFP Antibody

Immunoprecipitation: GFP Antibody [NB600-303]

Immunoprecipitation: GFP Antibody [NB600-303] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.

Western Blot: GFP Antibody [NB600-303]

Western Blot: GFP Antibody [NB600-303] - Analysis of GFP HRP expression in HeLa/GFP cell lysate.

Western Blot: GFP Antibody [NB600-303]

Western Blot: GFP Antibody [NB600-303] - Analysis using Biotin conjugate of NB600-303. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

Immunohistochemistry: GFP Antibody [NB600-303]

Immunohistochemistry: GFP Antibody [NB600-303] - Eight-week-old Lgr5-GFP mice were injected with CCL4 and rHGF or/and rRspo1 proteins (0.1 ug/mice/time), as indicated in a, Lgr5 expression was measured using an immunofluorescent assay with anti-GFP antibody. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-017-01341-6) licensed under a CC-BY license.

Western Blot: GFP Antibody [NB600-303]

Western Blot: GFP Antibody [NB600-303] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303]

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303]

GFP Antibody

Western Blot of GFP Antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.

GFP Antibody

Western Blot of Rabbit anti-GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 () at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Western Blot: GFP Antibody [NB600-303] -

The polo-boxes of Plk4 are not sufficient to mediate STIL binding.(A) Scheme of Flag-Plk4 fragments. (B) After overexpression of Flag-Plk4-fragments (A) and GFP-STIL in HEK293T cells, cell lysates were subjected to immunoprecipitations using anti-Flag antibodies. Coprecipitation of GFP-STIL with Flag-Plk4 fragments was detected by western blotting using anti-GFP and anti-Flag antibodies.

Western Blot: GFP Antibody [NB600-303] -

Western Blot: GFP Antibody [NB600-303] - The polo-boxes of Plk4 are not sufficient to mediate STIL binding.(A) Scheme of Flag-Plk4 fragments. (B) After overexpression of Flag-Plk4-fragments (A) & GFP-STIL in HEK293T cells, cell lysates were subjected to immunoprecipitations using anti-Flag antibodies. Coprecipitation of GFP-STIL with Flag-Plk4 fragments was detected by western blotting using anti-GFP & anti-Flag antibodies. Image collected & cropped by CiteAb from the following publication (https://journals.biologists.com/bio/article/4/3/370/1390/Plk4-dependent-phosphorylation-of-STIL-is-required), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303] -

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303] - Lgr5+ liver stem cells transplantation decreased liver fibrosis. a Schematic overview of the experimental setup. Eight-week-old wild-type C57 mice were i.p. injected with CCL4 (2 ml/kg, Sigma-Aldrich) dissolved in olive oil at a ratio of 1:4, or olive oil alone (2 ml/kg) twice a week for 6 weeks. Lgr5-GFP+ liver stem cells or primary hepatocyte (PH) derived from Lgr5-GFP mice were transplanted into the liver fibrosis mice by intrasplenical injection on day 0. b Lgr5+ cells were isolated from Lgr5-GFP mice treated with CCL4 by FACS assay for transplantation (left). The liver Lgr5 expression was stained using anti-GFP antibody in mice with Lgr5+ cells or PH transplantation (right) on day 40. n = 10 mice, scale bars, 200 μm. c, d Lgr5+ liver stem cells transplantation decreased CCL4-induced liver fibrosis & recovered liver functions. Lgr5-GFP+ liver stem cells or PH transplantation were described in a; the livers were harvested & stained using H&E & Sirius Red for fibrosis analysis & quantification of positive-staining areas measured by Image J software (c). The serum was harvested for ALT & AST analysis (d, e). For c, scale bars, 200 μm, the results are shown as mean ± s.d. of five independent sections taken randomly per mice & a total of 50 tissue specimens in each group (n = 10 mice) *p < 0.05, **p < 0.01. For d, e, triplicates for each condition were analyzed. The results are shown as mean ± s.d. of three independent experiments. *p < 0.05, **p < 0.01 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29079780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.