GFP Antibody [PE/Atto594]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-308PEATT594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Applications
Dot Blot, Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot
Label
PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for GFP Antibody [PE/Atto594]
Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Reactivity Notes
No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP.
GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725).
Mouse reactivity reported in multiple pieces of scientific literature.
Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099).
Use in Mouse reported in secitific publication (PMID: 32765228).
Plant reactivity reported in scientific literature (PMID:32896843)
GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725).
Mouse reactivity reported in multiple pieces of scientific literature.
Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099).
Use in Mouse reported in secitific publication (PMID: 32765228).
Plant reactivity reported in scientific literature (PMID:32896843)
Specificity
No reaction was observed against Human, Mouse or Rat serum proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for GFP Antibody [PE/Atto594]
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Electron Microscopy
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Whole-Mount
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: GFP
References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001
2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075
3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9
Long Name
Green Fluorescent Protein
Alternate Names
eGFP, GFPuv
Additional GFP Products
Product Documents for GFP Antibody [PE/Atto594]
Product Specific Notices for GFP Antibody [PE/Atto594]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...